Polyphenol-Mediated Antibody Functionalization of Titanium Peroxide Nanoparticles for Cancer Cell Targeting

Abstract

Cell targeting would benefit various biotechnological applications such as disease diagnosis and cancer therapy. However, efficiently functionalizing nanoparticles with targeting ligands such as antibodies remains challenging. For example, poly(acrylic acid)-modified titanium peroxide NPs (PAATiOx) have shown promising radiosensitizing effects but suffer from poor tumor accumulation due to a lack of targeting. Herein, we developed a simple, one-pot process to noncovalently graft anti-CD44 antibodies onto the surface of PAATiOx NPs using tannic acid, a polyphenol that can bind to diverse surfaces and biomolecules via multiple molecular interactions. We evaluated the cellular binding, internal..